UZH/ETH - FGCZ - In situ Synthesis of Oligo Arrays

Light directed in situ oligonicleotide synthesis is performed from scratch in 3D microchannels allowing the user maximal flexibility and speed. 1) Spacers are bound to activated surface, 2) protection groups are cleaved at illuminated spots 3) binding of amidites results in oligo elongation, 4) capping of un-elongated oligos prevents the synthesis of false oligos. Step 2 to 4 are repetet until the desired oligo length is achieved.

Geniom one is a DNA/RNA technology platform that is used in Gene Expression Profiling, Epigenetic Profiling (DNA methylation), SNP Typing, microRNA analysis, ChIP on chip assays. It enables the design, synthesis, and analysis of user-specified biochips (DNA arrays). Within a single day DNA/RNA information is translated into microarray experiments and results.

System Characteristics

Two Geniom System Ones at FGCZ

Geniom System One with open front door

Microarray capacity	A single Biochip with 8 separate microarrays, each containing 6,000 features. Expansion on 15,000 features is planned for 2007
Probe length	User selectable. Current Standard Synthesis Kits support 30mer oliogonuceleotides. is available
Sample throughput	Typically 8-16 samples per day; depending on application
Detection	Fluorescence detection, 8 megapixel CCD camera, automated feature extraction. 3 standard filter positions: Cy3, Cy5 and FRET (ex Cy3, em Cy5); additional filters on request
Processing times	Array design and editing within minutes; overnight array production; hybridisation 1-16 hours depending on application
Synthesis kits	5'-Standard Synthesis Set (up to 30mers), Longmer synthesis kit (up to 60mer) for synthesis of oligonucleotides with uncapped 5' ends. 3'-Inverse Synthesis Set: for inverse oligonucleotide synthesis with uncapped 3' end

For detailed Information please visit www.geniom.com

Contact

Andrea Patrignani
Stefan Neuenschwander

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