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Mass Spectrometers
| Applications | Specifications | |
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AB Sciex 5600 TripleToF Bernd Roschitzki |
Gel / LC-MS/MS LC-MS/MS (MudPIT) Intact proteins Labeled and label-free quantitative proteomics |
Hybrid mass spectrometer Quadrupole–Time-of-Flight Mass Analyser High scan speed (up to 50 MS/MS per sec) with high resolution (>20’000 FWHM) and high mass accuracy (2ppm) Fragmentation: CID HPLC: Eksigent UPLC 1D plus Software: Analyst TF |
|
AB Sciex 5500 Q-TRAP Asa Wahlander |
Targeted analysis (SRM/MRM) Neutral loss scan analysis Precursor scan analysis LC-MS/MS (MudPIT) Absolute and relative quantification |
Hybrid mass spectrometer High sensitivity instrument Triple Quadrupole Linear Accelerator Trap Fragmentation: CID HPLC: Eksigent UPLC 1D plus Software: Analyst |
|
LTQ-Orbitrap Velos Asa Wahlander |
Gel / LC-MS/MS; LC-MS/MS (MudPIT) top-down proteomics high accuracy MS/MS PTM detection Labeled and label-free quantitative proteomics |
Hybrid mass spectrometer Dual-Pressure Linear Ion Trap and Orbitrap Resolution power: >100’000 @ 400 m/z Mass accuracy: <3 ppm (extern. calibration) <1 ppm (intern. calibration) Fragmentation: PQD, CID, HCD HPLC: Eksigent UPLC 1D plus with cHiPLC Software: Xcalibur |
|
LTQ-Orbitrap Velos ETD Paolo Nanni |
Gel / LC-MS/MS; LC-MS/MS (MudPIT) top-down proteomics high accuracy MS/MS PTM detection Labeled and label-free quantitative proteomics |
Hybrid mass spectrometer Dual-Pressure Linear Ion Trap and Orbitrap Resolution power: >100’000 @ 400 m/z Mass accuracy: <3 ppm (extern. calibration) <1 ppm (intern. calibration) Fragmentation: PQD, CID, HCD, ETD HPLC: Eksigent UPLC 1D plus with cHiPLC Software: Xcalibur |
|
Thermo TSQ Quantum Endre Laczko |
SRM Metabolome analysis |
Triple Quadrupole Mass Spectrometer |
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Thermo TSQ Vantage Endre Laczko |
SRM Metabolome analysis |
Triple Quadrupole Mass Spectrometer |
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Waters GCT Premier Endre Laczko Stefan Schauer |
Metabolome analysis | GC-TOF Mass Spectrometer |
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LTQ-FT-ICR Ultra Bernd Roschitzki |
Gel / LC-MS/MS LC-MS/MS (MudPIT) top-down proteomics high-resolution MS PTM detection, SILAC, ICAT, ICPL, label-free proteomics |
Hybrid mass spectrometer 2D ion trap and Fourier transform ion cyclotron resonance MS (ICR) Resolution power: 1’000’000 @ 400 m/z mass accuracy: <2 ppm (extern. calibration) Fragmentation: PQD, CID HPLC: Eksigent 2D Autosampler: Spark Holland Software: Xcalibur |
|
LTQ-Orbitrap XL ETD Paolo Nanni |
Gel / LC-MS/MS; LC-MS/MS (MudPIT or RP/RP) top-down proteomics high accuracy MS/MS Electron Transfer Dissociation (ETD) PTM detection ICAT, iTRAQ, label-free proteomics |
Hybrid mass spectrometer 2D ion trap, Orbitrap and HCD collision cell; Resolution power: 100'000 @ 400 m/z Mass accuracy: <5 ppm (extern. calibration) <1 ppm (intern. calibration) Activation: ETD, HCD, PQD and standard CID Pump: Eksigent 1D plus Autosampler: Spark Software: Xcalibur |
|
LTQ-Orbitrap Peter Gehrig |
Gel / LC-MS/MS; LC-MS/MS (MudPIT) top-down proteomics high accuracy MS/MS PTM detection ICAT, Silac, label-free proteomics |
Hybrid mass spectrometer 2D ion trap and Orbitrap Resolution power: 100’000 @ 400 m/z Mass accuracy: <5 ppm (extern. calibration) <1 ppm (intern. calibration) Activation: PQD, CID Pump: Eksigent 1D plus Autosampler: Spark Software: Xcalibur |
|
Waters QTOF G2 HDMS Endre Laczko |
Metabolome analysis, lipid analysis, analysis of whole protoeins, analysis of non-covalent protein-ligand and protein-protein interactions, MS^e analysis, label-free proteomics |
Resolution power: 40’000 Mass accuracy: 1 ppm Ion mobility separation Activation CID Pump: Waters nanoAcquity UPLC Software: MassLynx, ProteinLynx Global Server |
|
LTQ Bernd Roschitzki |
Gel / LC-MS/MS; LC-MS/MS (MudPIT) High throughput proteomics (C-MOP) large scale analysis |
2D linear ion trap high scan rate Pump: Ultimate Autosampler: FAMOS Software: Xcalibur |
|
ABI 4800 MALDI TOF/TOF™ Peter Gehrig |
- Analysis of complex proteome samples by liquid chromatography - MALDI mass spectrometry (LC-MALDI-MS) - Peptide and protein quantification using iTRAQ reagents - Studies of protein phosphorylation and of other post-translational modifications - Structural characterization of oligosaccharides, glycans, lipids and of other biomolecules |
Tandem time-of-flight mass spectrometer Detection limit: Medium attomole range Mass accuracy: < 35 ppm (ext. calibration) < 10 ppm (int. calibration) Software: 4000 Series Explorer |
|
Bruker Ultraflex Protein Service: Rene Brunisholz |
Mass determination of intact biopolymers Protein identification of low complex samples - peptide mass finger printing (PMF) - MALDI-MS/MS Protein identification of complex samples - LC-MALDI-MS/MS Structural analysis (MS/MS) of peptides Top-down proteomics Carbohydrate analysis |
Smart beam upgrade HPLC: Agilent 1100 capLC, Agilent microspotter Software: FlexControl, FlexAnalysis, Biotools |
|
Bruker Autoflex Protein Service: Peter Hunziker |
Mass determination of intact biopolymers Protein identification of low complex samples - peptide mass finger printing (PMF) |
Software: FlexControl, FlexAnalysis, Biotools |
|
Waters QTOF Ultima API Protein Service: Peter Hunziker |
Mass determination of intact biopolymers Mass determination of small molecules Structural analysis (MS/MS) of peptides and small molecules |
HPLC: Waters nano Aquity UPLC Software: MassLynx, BioLynx, ProteinLynx Global Server |
|
Waters QTOF G2 HDMS UserLab: Endre Laczko Protein Service: Peter Hunziker |
Protein identification of complex samples - LC- MS/MS (“shotgun proteomics”) - Gel-LC-MS/MS Protein identification of low complex samples - LC-MS/MS - Gel-LC-MS/MS Structural analysis (MS/MS) of peptides |
HPLC: Waters nanoAquity UPLC Software: MassLynx, BioLynx, ProteinLynx Global Server |
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