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Amino Acid Analysis
AccQ-Tag and MassTrak running on an Acquity UPLC (Waters)
| Analysis | Minimum Recommended Amount | Optimal Amount | Notes |
| Amino acid composition and concentration of proteins/peptides (incl. hydrolysis in 6 M HCL) |
1 ug (lower amounts may still give some results but at a significantly reduced accuracy) |
2 – 10 ug |
Ideally, sample should be free of non-volatile salts, buffers, primary amines and detergents. A small amount of buffer is compatible with the pre-column reaction as long as it does not alter the pH of the reaction (40 ul 0.2 M borate, pH 8). Primary and secondary amines react with the reagent and their products may co-elute with amino acid derivatives (e. g. the reaction product of Tris co-elutes with Ser) |
|
Free amino acids (without hydrolysis) |
50 ul | 100 – 200 ul |
Volumes indicated are for mouse urine and plasma; they may be significantly different for other species or for tissue extract. Do not perform SSA precipitation, this should be carried out immediately before analysis. |
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