UZH/ETH - FGCZ - Mass Spectrometers

Hybrid mass spectrometer
2D ion trap and Fourier transform ion cyclotron resonance MS (ICR)
Resolution power: 500’000 @ 400 m/z
mass accuracy: <2 ppm (extern. calibration)
Activation: PQD, CID
Pump: Eksigent 2D
Autosampler: Spark
Software: Xcalibur

LTQ-Orbitrap XL ETD

Doro Rutishauser

Gel / LC-MS/MS;
LC-MS/MS (MudPIT or RP/RP)
top-down proteomics
high accuracy MS/MS
Electron Transfer Dissociation (ETD)
PTM detection
ICAT, iTRAQ

Hybrid mass spectrometer
2D ion trap, Orbitrap and HCD collision cell;
Resolution power: 100'000 @ 400 m/z
Mass accuracy: <5 ppm (extern. calibration)
<1 ppm (intern. calibration)
Activation: ETD, HCD, PQD and standard CID
Pump: Eksigent 1D plus
Autosampler: Spark
Software: Xcalibur

LTQ-Orbitrap

Doro Rutishauser

Gel / LC-MS/MS;
LC-MS/MS (MudPIT)
top-down proteomics
high accuracy MS/MS
PTM detection
ICAT, Silac

Hybrid mass spectrometer
2D ion trap and Orbitrap
Resolution power: 100’000 @ 400 m/z
Mass accuracy: <5 ppm (extern. calibration)
<1 ppm (intern. calibration)
Activation: PQD, CID
Pump: Eksigent 1D plus
Autosampler: Spark
Software: Xcalibur

Waters Q-Tof Ultima

Endre Laczko

Metabolome analysis,
Analysis of non-covalent protein-ligand and protein-protein interactions

Resolution power: 10’000 @ 400 m/z
Mass accuracy: 3 to 10 ppm
Pump: Agilent 1100 Cap-pump
Autosampler: Agilent
Software: MassLynx

LTQ

Bernd Roschitzki

Gel / LC-MS/MS;
LC-MS/MS (MudPIT)
High throughput proteomics
(C-MOP)
large scale analysis

2D linear ion trap
high scan rate
Pump: Ultimate
Autosampler: FAMOS
Software: Xcalibur

ABI 4700 MALDI TOF/TOF™

Peter Gehrig

Identification of proteins from 1-D and 2-D gels
Analysis of protein digests by liquid chromatography
- MALDI mass spectrometry (LC-MALDI-MS)
- Structural characterization of oligosaccharides, glycans, lipids and of other biomolecules

Tandem time-of-flight mass spectrometer
Detection limit: High attomole range
Mass accuracy: < 50 ppm (ext. calibration)
< 10 ppm (int. calibration)
Software: 4000 Series Explorer

ABI 4800 MALDI TOF/TOF™

Peter Gehrig

- Analysis of complex proteome samples by liquid chromatography
- MALDI mass spectrometry (LC-MALDI-MS)
- Peptide and protein quantification using iTRAQ reagents
- Studies of protein phosphorylation and of other post-translational modifications
- Structural characterization of oligosaccharides, glycans, lipids and of other biomolecules

Tandem time-of-flight mass spectrometer
Detection limit: Medium attomole range
Mass accuracy: < 35 ppm (ext. calibration)
< 10 ppm (int. calibration)
Software: 4000 Series Explorer

Bruker Ultraflex

Protein Service:
Rene Brunisholz

Mass determination of intact biopolymers
Protein identification of low complex samples
- peptide mass finger printing (PMF)
- MALDI-MS/MS
Protein identification of complex samples
- LC-MALDI-MS/MS
Structural analysis (MS/MS) of peptides
Top-down proteomics
Carbohydrate analysis

Smart beam upgrade
HPLC: Agilent 1100 capLC, Agilent microspotter
Software: FlexControl, FlexAnalysis, Biotools

Bruker Autoflex

Protein Service:
Peter Hunziker

Mass determination of intact biopolymers
Protein identification of low complex samples
- peptide mass finger printing (PMF)

Software: FlexControl, FlexAnalysis, Biotools

Waters QTOF Ultima API

Protein Service:
Peter Hunziker

Mass determination of intact biopolymers
Protein identification of complex samples
- LC- MS/MS (“shotgun proteomics”)
- Gel-LC-MS/MS
Protein identification of low complex samples
- LC-MS/MS
- Gel-LC-MS/MS
Mass determination of small molecules
Structural analysis (MS/MS) of peptides and small molecules

HPLC: Waters nano Aquity UPLC
Software: MassLynx, BioLynx, ProteinLynx Global Server

Wichtiger Hinweis:
Diese Website wird in älteren Versionen von Netscape ohne graphische Elemente dargestellt. Die Funktionalität der Website ist aber trotzdem gewährleistet. Wenn Sie diese Website regelmässig benutzen, empfehlen wir Ihnen, auf Ihrem Computer einen aktuellen Browser zu installieren. Weitere Informationen finden Sie auf
folgender Seite.

Important Note:
The content in this site is accessible to any browser or Internet device, however, some graphics will display correctly only in the newer versions of Netscape. To get the most out of our site we suggest you upgrade to a newer browser.
More information