Next Generation Sequencing Platforms
All numbers and specifications as of November 2011 - please check the Wiki page for latest information and updates !
|
|
Applications
|
Specifications
|
Input Material
|
Life Technologies 5500xl
|
SAGE/small RNA
Whole transcriptome
ChIP-seq
Methylation
Whole genome resequencing
Targeted resequencing
|
Throughput: 150 Mio reads/lane
up to: 2 Gigabases per run
Read-length:
Fragment: 75 bp
Mate-pair: 2 x 60 bp
Paired-end: 75 + 35 bp
Parallel and independent processing of up to 12 lanes. Multiple applications can be processed within the same run.
Barcodes available for the parallel measurement (multiplexing) of up to 96 samples
|
DNA Fragment library - 500ng of high quality column purified genomic DNA
Mate Pair library- 5-20µg of high quality column purified genomic DNA
Targeted Enrichment (Sure Select) - 3µg of high quality column purified genomic DNA
Chip-Seq - 20ng-1µg DNA
RNA seq - 200ng of poly(A) RNA or 200ng of rRNA-depleted RNA
small RNA analysis - 5-10µg of integral high quality total RNA
|
|
Pacific Biosciences RS
|
Backbone sequecning
de novo sequencing
Methylation studies
|
Throughput: 40 000 reads
Read length: Average ~1500 bp, individual reads up to 10000 bp
|
500bp library - 1µg of high quality column purified genomic DNA
2000bp library - 3µg of high quality column purified genomic DNA
8000bp library - 8µg of high quality column purified genomic DNA
1 library prep. produces enough material to sequence at least 2 SMRT cells
|
Illumina HiSeq 2000
|
SAGE/small RNA
Whole transcriptome
de novo sequencing
ChIP-seq
Methylation
Whole genome resequencing
Targeted resequencing
|
Throughput: 3000 Mio reads
up to 600 Gigabases per run
Read length: 2 X 100 bp
|
DNA Seq - From 1 μg high-quality
RNA-free genomic DNA
RNA Seq - From 100 ng to 4 µg column purified, genomic DNA free total RNA
Small RNA Seq - From 1 to 10 μg of trizol purified total RNA
Chip Seq -From 10 ng Single ChIP enriched DNA or input DNA
|
Ion Torrent PGM
|
Amplicon sequencing
Small genome sequencing
Small RNA
|
Throughput:
> 10 Megabases (314 chip)
> 100 Megabases (316 chip)
Read length: 200 bp
|
DNA Seq - From 100 ng to 5 μg high-quality RNA-free genomic DNA
RNA Seq - From 200 ng to 1 µg poly(A)RNA or rRNA-depleted total RNA
|
Roche 454 GS FLX+
|
de novo genome sequencing
de novo transcriptome sequencing
Whole genome resequencing
Fragment/amplicon deep sequencing
|
Over 1 Million reads per run
up to 700 Million bases per instrument run
Average read length of 700 bases
Maximum read length of 1000 bases
single read accuracy greater than 99.5%
consensus accuracy greater than 99.99%
|
1ug for an average read length of 700bp
500ng for a shotgun fragment library of 400bp read-length
For mate pair libraries, with insert sizes of 3 kb, 8kb or 20kb, we need a input of gDNA of 5ug, 15ug or 30ug, respectively.
For RNA analysis, 1 to 2 ug of total RNA.
|
E-mail contact: sequencing@fgcz.ethz.ch
