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Mass Spectrometry
News & Events
- A variety of applications running on MALDI-TOF (Bruker Autoflex II), MALDI-TOF-TOF (Bruker Ultraflex II with Smartbeam) and ESI-MS-MS (Waters Synapt G2)
| Analysis | Minimum Recommended Concentration | Optimal Concentration | Notes |
|
Mass determination (MALDI-MS, ESI-MS) |
1 – 5 pmol/ul | 10 – 500 pmol/ul |
Minimum submitted volume above 5 ul. Volatile solvents free of non-volatile buffers, salts, detergents, glycerol, etc. Salt and buffer containing samples may be submitted (we will desalt them on ZipTips prior to the analysis). For protein measurements, as a rule of thumb concentrations of (mass in kDa) x 1 pmol/ul are optimal |
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| Analysis | Minimum Recommended Amount | Optimal Amount | Notes |
|
Identification of unknown proteins, confirmation of protein identity (MALDI-MS, MALDI-MS-MS, LC-MALDI-MS-MS, LC-ESI-MS-MS) |
50 – 250 fmol | 1 pmol | Submit samples as Coomassie Blue stained gel bands (whenever possible avoid silver staining) or in solution (free of protease inhibitors!) |
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