FAQ Proteomics

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Q: How should I provide my sample for mass determination and/or structural analysis?

A: For these type of analysis, your samples should fulfill these criteria:

  • minimal volume: above 5 µl
  • minimal recommended concentration: 1-5 pmol/µl
  • optimal concentration: 10-500 pmol/µl
  • dissolved in volatile solvents
  • free of non-volatile buffers, salts, detergents, glycerol, etc.
  • salt and buffer containing samples may be submitted (we will desalt them on ZipTips prior to the analysis)
  • for protein measurements, as a rule of thumb concentrations of (mass in kDa) x 1 pmol/µl are optimal

Q: How should I provide my sample for protein identification analysis?

A: For the identification of unknown proteins or confirmation of protein identity using MALDI or ESI mass spectrometry, your samples should fulfill these criteria:

  • submit samples as Coomassie Blue stained gel bands (whenever possible avoid silver staining) or in solution (free of protease inhibitors!)
  • minimal recommended amount: 50 – 250 fmol
  • optimal amount: 1 pmol

Q: How should I provide my sample for amino acid analysis (incl. Hydrolysis)?

A: For these type of analysis, your samples should fulfill these criteria:

  • minimal volume: 5 µl
  • minimal amount: 1-5 µg
  • optimal concentration: 2-10 µg
  • dissolved in volatile solvents
  • free of non-volatile buffers, salts, detergents, primary amines, etc.
  • small amounts of buffer is compatible with the pre-column reaction as long as it does not alter the pH of the reaction (40 ul 0.2 M borate, pH 8)
  • Primary and secondary amines react with the reagent and their products may co-elute with amino acid derivatives (e. g. the reaction product of Tris co-elutes with Ser)

Q: How should I provide my sample for analysis of free amino acids?

A: For these type of analysis, your samples should fulfill these criteria:

  • minimal volume: 50 µl
  • optimal volume: 100-200 µl
  • Volumes indicated are for mouse urine and plasma; they may be significantly different for other species or for tissue extract.Do not perform SSA precipitation, this should be carried out immediately before analysis.

Q: How should I provide my sample for sequence analysis of proteins and peptides?

A: For these type of analysis, your samples should fulfill these criteria:

  • minimal volume: above 5 µl
  • minimal recommended amount: 2 pmol
  • optimal amount: 10-50 pmol
  • Samples in solution: Solvent free of primary amines such as Tris or Gly
  • Samples on PVDF membrane: Maximum membrane size is 15 – 20 mm2, whenever possible avoid Tris/Gly buffer for electroblotting

 

Q: How can I send you my samples?

A:You can send us samples in any mode. You could step by and give them to us personally, or send them using internal post, normal post, velo couriers or any other means. Obviously, not via FAX! :-)

Q: How long do I have to wait until I receive my results?

A: If not stated differently, results will normally be delivered within 5 working days for service analysis. However, processing speed depends on availability of machine time and sample queue of already running services.

 

 
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24.04.2017
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